FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1591749
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32480.20405227206048188No Hit
TCATTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT16210.1018376641040767No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT16120.101272248325584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGTA12600.026.38958770
TCTAGGG5500.022.9161782
GTCTAGG2950.022.5528891
CTTTTTT31750.022.377149
ATCTTTT31850.022.3068837
TCTTTTT32350.021.853928
TTTCGGA4300.021.1726631
GATCTTT37700.021.0735056
GTACAAT1356.0027814E-620.7504161
GTATTAG1709.280484E-820.5978411
CTTGTGT16600.020.03065169
TAAGTTC8300.019.81917830
TTCGGAA5150.019.7148342
GTATATG1451.1131226E-519.3193531
TGTATAA2002.4450856E-819.24936
GTAAGTT8550.019.23967229
TTCTATC1107.4633065E-419.0968132
TGGCGTA7750.018.96705250
ATAGAGT3001.8189894E-1218.6659877
TAAGGTA10350.018.5995255
Produced by FastQC (version 0.11.5)