FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944710
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT67720.22997171198522096No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT45450.1543445704330817No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA44750.15196742633400234No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT44230.15020154786040052No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA43580.14799419976839825No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43120.14643207650328896No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG43030.14612644369055017No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC40850.13872333778198873No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG37890.12867141416302455No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT36500.12395108516628121No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC36340.12340773794363452No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA35270.11977410339218462No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT33210.11277850790060821No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA31870.108227974910942No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG29940.10167384903776602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAC11100.021.4467283
CGCGTAA15450.020.15993521
CGTAAAT15700.020.06250823
GTCCTAG23050.019.75061
TACTATA11950.019.6299362
TAGGGCA22100.019.32045228
GGTCCAA17500.018.59921611
TCCTAGA25700.018.527542
GTCGGTA21000.018.49984754
CAGACAT23150.018.44445833
ATATAAG12550.018.4124762
ATACCTT11800.018.3903036
GCGCGTA17150.018.36563720
TACCTTT11450.018.3407737
TGATAGG24450.018.17888325
CTATAGG1351.4074254E-418.1581751
CTATACT4050.018.1501564
AGACATT23400.018.09783434
TAAATTA17300.018.00471325
GTCTTAC21650.017.9544851
Produced by FastQC (version 0.11.5)