FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650064
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACG38050.2305971162330673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34460.20884038437296976No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTACGTAT25520.15466066770743436No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA25110.15217591560084942No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA22070.1337523877861707No Hit
GCTCTAGTAACGGCGAGTGAAGCAGCAATAGCTCAAATTTGAAATCTGGC20090.12175285322266287No Hit
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACT19140.11599550078057579No Hit
CTCTATGACGTCCTGTTCCAAGGAACTTAGGTCGGTGGCCACTCAGAAGC19130.11593489707065907No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTG400.002419527235.000625
TTTCGGA2650.029.0668071
ACGTGTA10050.025.4271970
CTAGTCG14100.024.8208820
ATCTTTT19800.024.3936317
GATCCCT15050.024.1850115
TAGTTTA14050.024.16339730
CCTAGTC14700.024.04658919
TACTAAC15500.023.7180041
GATCTTT24500.023.713996
GTCTAGG2250.023.3415281
GAATAAT7950.023.3415281
TCTTTTT20150.022.9277528
ATAGTTT15300.022.87552829
AGTTTAT15000.022.8663831
CTTTTTT20400.022.6460889
CTACTTG9450.022.593683
GTCCGAT17050.022.57967811
CGGCATA15600.022.43561725
TACTTGT9700.022.371534
Produced by FastQC (version 0.11.5)