FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856730
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23630.2758161847956766No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACT2650.033.024653
ATAACTT2750.031.8218984
ACATATC500.007211413227.99836553
TGTCATT2950.027.28654338
TAATACT906.167553E-627.22544
TTTGGTC3300.026.5151529
GGTCTGA3350.026.11940212
CATACGT959.381445E-625.793992
TTGTCAT3200.025.15478137
AGACTTA1301.6075501E-724.22935550
GTCATTA3350.024.02844839
ATTATCA3500.023.99859842
TATTTGT3700.023.6472719
AATTCGT3450.023.3319754
TTTGTCA3500.022.99865736
CTTTTTT15100.022.947029
TTGGTCT4000.022.75000210
TTTCAAT3550.022.67473250
TCTTTTT15350.022.5732888
TATTTCG1403.284913E-722.56
Produced by FastQC (version 0.11.5)