FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1729936
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA27430.1585607791270891Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC25730.14873382599125054Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23420.1353807308478464No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC22870.13220142248036923No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC18900.10925259662785211No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC18860.10902137420112652No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC17950.10376106399311881No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4150.029.5319231
ATCTTTT33700.026.0691767
TCTTTTT33100.026.013018
CTTTTTT33650.025.6911059
GAGTGTA12850.025.05997870
GAGCATA2300.024.3592531
GATCTTT45650.022.2358256
AGCATAC2259.094947E-1221.7835882
TTCGGAA5800.021.1263242
CATACGT2252.1100277E-1020.224114
GACGGAG72700.019.59548670
ACTGTCG1451.117361E-519.3110318
ACCGTTA3350.018.8066378
AGAGTGT17750.018.73360369
GTCATAA1551.9775458E-518.0729921
AGTGTAG10700.017.99181469
GGTTAGG2751.6007107E-1017.8265441
CGACGGA80850.017.75006369
GTACAGG6000.017.5082111
TAGGGGT5200.017.5016334
Produced by FastQC (version 0.11.5)