FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186744
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34950.29450327956155664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTG7100.029.578877
GTCTTAG500.0071880528.017861
TTTCGGA7400.027.9232061
CGGTGTA13450.027.06790470
ATCTGAG8100.025.927167
TCTGAGC5050.025.6447858
ATCTTTT61800.025.5432857
GATCTTT66100.025.2054336
TCTTTTT62550.025.1810538
CTTTTTT64000.024.5548239
CGATCTG112150.023.5633135
GAATTAT2900.022.93502470
GATCTGG41850.022.4980836
TTCGGAA9650.022.4975972
CTAGGCC2207.2759576E-1222.2728489
CGATCTT100950.022.0862435
TATTAGT800.00265994521.8852622
TCTGTGC4350.021.7251728
ACGGTGT17450.021.06383569
ATCTGGG16850.020.357057
Produced by FastQC (version 0.11.5)