FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1548402
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21210.13697993156815866No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA16150.10430107943544377Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC15980.10320317333612332Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTA604.98589E-429.1658637
TAGGGGT5050.024.954644
TTTCGGA3950.024.8246731
GTGTAGT1601.8535502E-924.0766071
ATCTTTT28900.022.8886417
TCTTTTT28300.022.7555528
CTTTTTT28350.022.5919679
TAGACGG2259.094947E-1221.7799875
AGGTGTA12300.021.62611870
GATCTTT35050.020.8702816
CATACGT1356.0064613E-620.7488782
GATTAGT1205.7205536E-520.41610115
TTCGGAA5000.020.3079642
GTCTAGG3700.019.876511
GAAGGCT60300.019.50257770
TTTATAC900.00527064219.448933
GTCGTTA2053.2448952E-818.77996821
TAATTTC2801.0913936E-1118.7494836
GAGCGTA3101.8189894E-1218.06401611
GGTTAGG2601.4497346E-917.510261
Produced by FastQC (version 0.11.5)