FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453542
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22970.15802776940741994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGTA10000.024.150570
ATCTTTT26350.023.9085887
CTTTTTT25950.023.8716859
TCTTTTT26150.023.5560828
TATCATA750.001832676123.33461868
TTTCGGA4900.022.8709891
GATCTTT32250.021.9229326
TATTGTA800.00266184121.882982
TTAGAAT2001.0659278E-921.0062123
GCCTAAC1257.831077E-519.59905621
CTTGTGT12400.019.47620869
GCCTTAT1801.6927879E-719.4562241
GTAGTAA950.007186343418.4322131
ATACCGT1903.0077354E-718.4214346
TACTGGA2104.256617E-818.3400212
CTGGGGT4400.018.298354
ATCTGGG17050.018.2695437
GATACTC47150.018.26124670
GTTTATG1602.5946463E-517.5106031
GTTCAAA1803.6386991E-617.5106031
Produced by FastQC (version 0.11.5)