FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2993645
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42170.1408650658311189No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA30550.10204950820822108Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA500.007197322328.0118392
TTTCGGA9550.026.769711
ATCTTTT51500.023.378767
TCTTTTT50400.023.124738
CTTTTTT53300.021.8658079
GATCTTT65700.021.2028966
TTCGGAA12200.020.9514772
GTGTGTA22250.019.82231170
CATACGT3250.018.3096184
AGCATAC3101.8189894E-1218.0721532
GATATGC102150.017.9558870
GCCTTAT4150.017.7212921
GAGCATA3551.8189894E-1216.770451
TACTGGA3551.8189894E-1216.7676492
TTATTCC3207.8216544E-1116.4079784
ATCTGTG25200.016.3889717
GTATTGA2803.8871804E-916.2595861
AGTGTGT28150.016.1651169
TAGGGGT10600.015.8507254
ATCTGGG34700.015.7349497
Produced by FastQC (version 0.11.5)