FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047439
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34370.16786824906627254No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTA703.488006E-530.0052365
ATCTTTT59200.028.8514527
GATCTTT65900.028.0432726
CTTTTTT61800.027.3537969
TCTTTTT61900.027.2530638
TTTCGGA9200.026.2661291
ATCTAGC10900.026.0092667
TTGTGTA24900.022.91508570
ATCTGAG13900.022.9137487
ATCTAGG7700.022.727357
TCTAGCC5100.022.6465858
TCTAGGC4150.021.927258
ATCTGTG11350.021.894357
CGATCTT131600.021.8389175
GATCTGG58900.021.571066
CGATCTG170750.021.5059495
ATCTGGG22350.021.1410127
TTCGGAA11200.020.9452462
TCTAGCG4550.020.7687978
CTTGTGT30000.018.78615269
Produced by FastQC (version 0.11.5)