FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794634
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19370.24376002033640645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGTG657.9513114E-426.9264665
TACACTC700.00122532225.0031455
TTTCGGA3400.024.7198811
ACGTGTA11900.024.41330170
ATCTTTT14450.023.494817
TAGGGGT2250.023.340684
TACATTA750.001829182623.3406774
CTTACTC1052.0346415E-523.3406773
TAGACTC901.9264994E-423.336275
CTTTTTT14900.022.5503359
TCTTTTT14950.022.4749188
TCTGAGC2650.022.4528318
GTATTGG1253.0748743E-622.4126931
TATTAGG1102.9106513E-522.2839472
ATCTGAG6350.022.0472437
GTGTAAT800.002657673321.8873961
GGTATTA800.002667403521.87362315
CGTGTAG7650.021.50597669
GATCTTT17550.021.3390316
GTACCGA1154.1174502E-521.304356
Produced by FastQC (version 0.11.5)