FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1231448
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24640.2000896505577174No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT22750.026.6159257
ATCTGTG6450.026.5896877
GATCTTT24300.026.36076
ATCTGGG12150.026.2145237
GATCTGG29600.024.9518576
TCTTTTT23450.024.9248668
CGATCTG75750.024.907835
CTTTTTT24200.024.584289
TCTGTGC4000.022.7495388
AGGTGTA8850.022.54740770
ATCTGAG5800.022.328047
TACACTT1304.339976E-621.5415235
CGATCTT48950.021.2389725
CTAACGT1205.710077E-520.4203994
TTCCGAT219350.020.049582
GTTACAC1055.455654E-420.0060923
TTACTCT1055.460136E-420.0036564
TTACACT1408.239007E-620.0036564
CCGATCT218200.019.9257324
ATAATAG3200.019.6934993
Produced by FastQC (version 0.11.5)