FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477586
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42800.28966165082776907No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA25260.17095451635302444Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC22630.15315521397739285Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC22310.15098951939176466No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC18020.1219556763531869No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC17730.11999301563496134No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC16600.11234540662946182No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTTG1305.456968E-1232.31353470
ATCTTTT24250.026.1240147
CGGGTAT4300.025.23114415
CTTTTTT25050.025.0094249
CGGGAAT3150.024.44307315
TCTTTTT25900.024.0535188
GGGTATT8400.023.74866916
GATCTTT31350.023.6693386
GAGTGTA9550.023.45973870
TTTCGGA3000.023.351781
CCGGGAA4850.023.0914914
CGGGAAC3000.022.16542415
TACTTGT1604.9039954E-821.8789564
GGTCCGG2451.8189894E-1221.4273711
ATCTGGG18300.021.4210137
ATAGCGT1003.933157E-421.0009546
GGTATTT9550.020.8888817
TAGGGGT4800.018.9617614
CCGGGTA4250.018.94011514
GATCTGG54400.018.7876196
Produced by FastQC (version 0.11.5)