FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175684
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22560.19188829651504996No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA20140.17130453421157385Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC19060.16211839235712996No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC18940.16109770992885844Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC17440.14833917957546416No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC16720.1422150850058349No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC15860.13490019426988883No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACAC400.002419780734.99904349
CTAGGCC1004.425856E-727.9992339
TTTCGGA2100.026.6795541
ATCTTTT18150.024.8753557
CTTTTTT17350.024.6102779
TCTTTTT17500.024.5993278
GTGATTA750.001833347523.3326939
GAATTAG35000.022.90034770
TCTAGGC2450.022.8565168
TAGTGAC1102.9241512E-522.27211834
ATACTGC800.002667606821.8744017
CATGAAT1454.6100286E-721.72354344
TAGCCAA1304.3460805E-621.53787210
CAATTAG1154.119129E-521.30467270
CTGGGGT2650.021.1332954
AATACTT1003.9344307E-420.99942616
TTCGGAA2351.6370905E-1120.8593652
CGAGATA2351.6370905E-1120.85138170
CGGTGTA6900.020.79741770
GATATGT3200.020.78068254
Produced by FastQC (version 0.11.5)