FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277779
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23560.18438243232984733No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA15290.1196607551071038Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC14410.112773805172882Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAT952.8204158E-729.47918970
AAGTATC1004.4180706E-728.0052368
CTTTTTT21900.027.645999
TCTTTTT21950.027.5851758
ATCTTTT22800.027.3243187
TTTAGTC1951.8189894E-1225.1260140
TTTCGGA3150.024.4624141
GATCTTT29600.023.6512156
CGTGTGG11300.022.92463370
GTATAAG1703.7289283E-922.6637081
TAGGTGT8200.022.19926869
TTAGTCT2301.2732926E-1121.3024941
TTCGGAA3950.021.2756232
GTATATA1003.9144905E-421.0154381
CTGAGTG1356.030079E-620.7389329
TGAGTGG1356.030079E-620.73893210
GTAATTA850.003774887920.603371
GTACACT1205.722172E-520.41488618
TTAGGTG9450.019.26285668
TACGTGT3650.019.18166268
Produced by FastQC (version 0.11.5)