FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367384
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31330.2291236404696852No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA15390.11255068071587791Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC15230.11138056317757118Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG1902.1827873E-1123.9610271
ATCTTTT27200.023.5474387
AGCATAC1652.6557245E-923.3389552
TTGTGTA9250.022.7023670
CTTTTTT27600.022.698939
TCTTTTT27900.022.5803038
TAGGGTC1102.9210232E-522.2756484
GTATAGT800.002658588621.8874761
CTTGTGT10000.021.6996769
GTGTAGG4200.021.6790241
GCTCTAA2108.367351E-1121.66633856
GAGCATA1807.225026E-921.4010891
TAGATAC1656.798109E-821.213355
GATACTC41050.020.20675870
GATCTTT35600.020.154196
TTTCGGA4200.020.0114061
TGTAGGA2101.9590516E-920.0048182
ACGTTAT1257.828655E-519.59970369
ACATAAT1451.1175687E-519.31005369
ACAGATA2607.2759576E-1118.84586766
Produced by FastQC (version 0.11.5)