FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2024879
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT60740.29996854133012396No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG60320.2978943433163167No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA56920.28110321653787707No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT49070.24233546794647978No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG44530.2199143751305634No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA44320.21887727612365976No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC43640.21551905076797181No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC41930.2070741017117566No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT40250.19877730965652762No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT36230.17892427152437257No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA34120.16850389578834093No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34110.16845451012134552No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG29980.14805822965224094No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT29820.14726805898031436No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA29750.1469223593113465No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA29050.14346536262166776No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC29000.1432184342866907No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA28740.14193440694481005No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA28280.13966266626302115No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA27240.13452655689549844No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA26370.13023000386689773No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT25910.12795826318510883No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC24640.1216862834766917No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA23960.11832805812100376No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA23950.11827867245400836No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA23940.11822928678701294No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT23070.11393273375841222No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA23020.11368580542343518No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA22930.11324133442047649No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC22300.11013003739976561No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG22270.10998188039877939No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC22230.10978433773079774No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA21780.10756198271600426No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCATAC1455.9299055E-1026.5652872
ATATAAG13250.025.3714432
GGTCCAA13750.025.1979311
AAGAATT14350.024.38823916
ACCGGTA14200.023.41298915
GTTATAC901.9245234E-423.3423673
AGAATTT15050.023.25390217
TATAAGA14400.022.8560683
GACGGTC15550.022.734628
CCGGTAA14700.022.6171916
CTCGCGT16150.022.32014729
CGGTCCA15550.022.05715410
CAAGAAT15600.021.9848315
TTGTCTC19450.021.95300710
CATACGT1604.8967195E-821.8829294
AGAAACG20850.021.8261467
TCGCGTC16200.021.81919330
CTCTCGC15950.021.72235127
CTTTTTT19700.021.4972869
AACGGCT21200.021.30120570
Produced by FastQC (version 0.11.5)