FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268211
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21960.8187583656151314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTT207.917766E-569.9996265
TTAATAT200.006946403552.49971857
TTTCGGA701.9126674E-840.014711
CATACTC350.00125408139.9997863
TTTATAC350.00125408139.9997863
CTGTATT400.002416202334.99981328
GATTATG400.002416202334.99981339
TAGCACG552.987476E-431.8180124
ATACATT552.987476E-431.8180123
AGCTCTC450.004302743431.1109435
GCATACG604.963533E-429.1773911
GGTTAGG500.007187756728.0102941
TAGACCC500.00720085827.999854
TGTATTG500.00720085827.9998529
TAGCCCG500.00720085827.9998510
CCATACT500.00720085827.999852
CATACGT500.00720085827.999852
TTACGCA500.00720085827.9998517
TTTGAAT500.00720085827.9998534
AGTAGGA500.00720085827.999852
Produced by FastQC (version 0.11.5)