FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621238
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37290.2300094125600313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA9450.027.7794470
CTTTTTT26400.026.7794289
ATCTTTT27100.025.9593647
TCTTTTT27950.025.4195658
GATCTTT32650.023.5834186
GTACGTA750.001833701423.33257343
TACGTAT952.7809606E-422.106594
TACTCTA2401.8189894E-1221.8749625
ATAGTGT13150.020.2293569
TTTCGGA5600.018.7632771
GTGTAAT1702.1065644E-618.5425341
GTCCTAA4750.018.4340951
ATCTGTG11400.018.4210197
ACTCTAC2306.0845196E-918.2608386
GATCTGG49450.017.9777246
GTCTAGG2952.1827873E-1117.8092121
ATCTGGG16550.017.552847
TCTAGGC2802.0736479E-1017.499438
CGATCTT79800.017.368395
TAAGGTA5500.017.181795
Produced by FastQC (version 0.11.5)