FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620377
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41300.2548789571809523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA5000.023.8127841
ATCTTTT32800.023.367757
CTTTTTT32550.022.4727049
TCTTTTT33050.022.0261428
GATCTTT41400.021.642116
AGCATAC1656.787741E-821.2169672
TCTGTAT6000.019.83235427
TACGTGT4650.019.57315668
ATACGTG4750.019.16108767
ATCTGGG20150.018.7583797
AGGTGTA11600.018.40824170
TAAAGTA1150.001008594818.25996850
TAGGCTC1150.001008594818.25996838
GATCTGG58650.018.0815076
CGTGTGG12400.018.06752670
TACACGT2207.276867E-817.5002174
GAAGATA4450.017.30625370
GATACGT4250.017.29699966
GGTATAC2451.3276804E-817.14465564
ATCTGTA17400.017.09685726
Produced by FastQC (version 0.11.5)