FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917872
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42760.46586016350863735No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.17322676800251016No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA12260.1335698223717468Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC11450.12474506249237366Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4750.037.598291
CGTTAGG708.662428E-735.0180171
TAGGGGT5100.035.0046584
CGCACTC400.002419540234.998947
TTCGGAA4950.033.955012
GAACGTA652.1047821E-532.3049518
GAGTGTA13150.031.94341570
CTAGGCC1001.2601959E-831.4990469
TTATACT703.486915E-530.0039944
GACGGAC604.979638E-429.17054770
GCCAATA500.0072126127.99762514
ATCTTTT107300.027.855637
GATCTTT110800.027.5758786
TCTTTTT108800.027.4072578
CATTAGG1154.877802E-827.4054031
CGTAATT906.1778555E-627.21991217
TATTTCG657.9595175E-426.922266
TTCGACA657.9620513E-426.92079412
CTTTTTT113050.026.376919
ACCTAGG808.638515E-526.2635121
Produced by FastQC (version 0.11.5)