FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373368
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19110.13914697298903136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA8650.028.72950670
ACGGTGT10200.024.70779669
CTTTTTT14900.022.080649
TCTTTTT15200.021.6448388
ATCTTTT16200.021.388997
GATCTTT20500.020.8293676
TCTGCGG3450.019.2754548
TCTAGGC1852.2691711E-718.9217623
ATCTGAG8750.018.8000877
TATAAGG1702.1114538E-618.53762
TGTATCA950.00719294818.4291922
GGTATAA950.007208077218.4224864
TAGACTA950.007209591618.421815
GTATAAG2104.2417014E-818.3454441
CAATTGA1351.412645E-418.14823347
AATTAGT1351.412645E-418.14823331
GATCTGG34300.018.0613086
TCTGAGT1953.9508996E-717.9488038
CGTTATA2751.6007107E-1017.81761610
GAATTAG38800.017.68113770
Produced by FastQC (version 0.11.5)