FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821025
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27850.1529358465699263No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTTT39100.028.7332158
ATCTTTT39700.028.6523827
CTTTTTT40800.027.4494639
GATCTTT46650.027.159696
AGGTGTA15050.025.81465170
TAGGTGT17350.022.39253669
TATGTAC800.00266392121.8803983
TTTCGGA5800.021.1328661
GATCTGG51050.020.019586
AGTAGTA1055.4551195E-420.0071322
TTATACT1751.2635974E-720.0043874
GTCTTAT1601.1806478E-619.698851
CTATTAG1501.4898373E-518.677431
TTCGGAA7550.018.0859182
CCTAATA1551.9795543E-518.0709612
TTAGGTG21950.018.01871168
CGATCTT108450.017.9457175
GAGCATA2155.5606506E-817.9173011
ATCTGGG20050.017.6309157
AGCATAC2005.135371E-717.5062432
Produced by FastQC (version 0.11.5)