FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417479
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21740.15337087886310838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT31650.027.867447
TCTTTTT32950.026.8741878
TTGTGTA12150.025.63889370
CTTTTTT34500.025.4629979
GATCTTT38600.024.6633386
ACTTACA1151.495413E-624.3531042
TACTATA1202.1654523E-623.338392
CTTGTGT13950.022.3306569
TTTCGGA5700.020.8898261
TTACACT1801.7021921E-719.4459154
GTTTATA900.005278383419.44385559
ATCTGAG11500.019.174017
TATGGTA1651.5941478E-619.09033411
CGATCTT80350.018.6006265
TAGTTAC2456.6211214E-1018.57086815
GTCTAGG1902.9891999E-718.4322011
TAACATG950.00721266918.42049610
GTCATAT2104.2447937E-818.3444271
AGTATAC1953.9423503E-717.952612
GATACTC51600.017.6363770
Produced by FastQC (version 0.11.5)