FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402197
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33520.23905342829859144No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG64450.031.39285
TTCCGAT152900.030.9143312
TAGTGTA7250.030.89951170
CCGATCT156900.029.694794
TCCGATC160250.029.5359043
CGATCTA27100.027.7711775
ATCTGTG5700.027.6322567
GATCTAG10150.027.244966
CTTCCGA179900.026.4739511
GATCTTT28250.026.145036
ATCTTTT26600.025.7901047
ATCTGAG6950.025.684087
GATCTGA18650.024.7753756
CTTTTTT27900.024.2103339
ATCTAGC4500.024.1117027
ATCTAGG3350.024.030447
GATCTGG33950.023.8175646
TCTTTTT28650.023.6995588
GATCTAA8500.022.6500366
CGATCTT52700.022.517195
Produced by FastQC (version 0.11.5)