FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014363
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36280.180106564705567No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT21290.1056909802255105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGTA13750.023.93144870
ATCTTTT32650.022.8330967
CTAGGGT1604.9090886E-821.8777314
GATCTTT38850.021.622716
TCTTTTT34700.020.9798418
CTATAGG2700.020.754151
GATCTGG50550.020.0801416
CTTTTTT36200.019.9166459
CGATCTT93200.018.7406795
TAGTACA1150.001007531318.263154
ATCTGGG20700.018.09187
ACCGTTA15500.018.064538
CTACTAG2551.1204975E-917.8546731
CGATCTG175150.017.8460465
ATCTGAG13500.017.3703827
GGACCGT16950.017.1395076
ATAGGGG9100.016.926033
TCTGAGC7900.016.8354578
CATACTG2108.58352E-716.667925
GACCGTT16650.016.6066177
Produced by FastQC (version 0.11.5)