FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269975
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24730.19472824268194255No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA19700.15512116380243707Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC17720.13953030571467942Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC16840.1326010354534538No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC13890.10937223173684521No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC13300.1047264709935235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT41500.028.252587
GAGTGTA10500.028.0017870
TCTTTTT41550.027.5447088
CTTTTTT42500.026.8455929
GATCTTT46650.026.1849846
TAGGGGT4500.024.1145444
TTTCGGA3950.023.9425411
AATTACG1055.467554E-419.99969548
TTCGGAA4750.019.9030532
CGATCTT96350.019.4355895
AGGGGTG14800.019.1566245
GGATACA1107.476496E-419.0913765
GAACGTA1107.4804574E-419.08986718
AGAGTGT15450.019.03033469
ATTACTC1301.0559551E-418.850323
GACGGAG61700.018.66407270
GAGCATA1702.104529E-618.5437341
CTAGGGG3400.018.5335083
CTAGGCG950.00721328118.4200469
AGTGTAG8200.018.35482469
Produced by FastQC (version 0.11.5)