FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790670
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC23400.130677344234281No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21770.1215746061530042No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC21060.11760960981085293No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA21060.11760960981085293Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC20040.11191341788269196Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC19600.10945623705093624No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC19210.10727828131369822No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT18950.10582631082220623No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC18580.10376004512277527No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA18200.1016379344044408No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT18000.10052103402637001No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTTT34250.024.3202178
CTTTTTT35350.023.861129
ATTGTAC1202.1660671E-623.3382853
ATCTTTT39450.022.5339747
GATCTTT45650.022.0041356
ATAGTGT850.003792437320.5874318
TCTAGGC2900.020.516448
ATCTGGG16350.019.9074787
CGTAAAT4800.019.6867323
ATCTGTG13050.019.309597
CACCTAG1107.4813183E-419.09016234
TAGGACT7750.018.9701774
CGTGTAG11100.018.91976569
CAGGGGT5800.018.7093014
TCTGAGC5450.018.6231258
GATCTGG47600.018.4556776
ACTATAC5150.018.3534093
TACTATA5250.018.0073412
GGTACAA5650.017.963957
TTTCGGA5100.017.8534071
Produced by FastQC (version 0.11.5)