FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295579
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31490.2430573511920153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT44800.029.4536117
TCTTTTT43850.029.452038
CTTTTTT45950.028.1049339
GATCTTT49950.027.6080636
AGGTGTA13900.026.19152670
TCTGAGC4700.024.5739258
TTTCGGA4900.023.5900291
AATAGTG800.002664682721.878745
CTATACT1304.3479013E-621.53715528
CGATCTT94900.021.0257245
TTCGGAA5500.021.0125122
TAGGTGT17550.020.74428669
ATCTGAG10200.019.902297
TCCTACA1107.447917E-419.1022832
AGTGTTA1107.469227E-419.09417370
CTACACA2801.0913936E-1118.753934
GATCTGG42400.018.7385186
TATACTG1702.121431E-618.52828829
TGCGTAT950.007212002818.4206478
ATCTAGC7300.018.219487
Produced by FastQC (version 0.11.5)