FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945133
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33240.17088805752614344No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA28830.14821608599514788No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC28310.14554274694840919No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT26690.13721426761049244No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT24680.12688078398752167No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC24140.1241046242082161No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT24020.12348769981281485No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG22310.11469652717834718No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA21440.1102238253116882No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT32700.026.3311947
TTGTGTA14500.024.86549670
TCTTTTT34750.024.3743258
CTTTTTT34850.024.1029039
GTTAGAG12800.021.60398362
CAGGGGT5500.021.0018144
ACGGTCC12900.020.8911279
GGTCCAA13350.020.7112711
AGGGGTG12850.020.7021775
CGGTCCA13750.020.61784210
GATCTTT46450.020.2703346
AGGTTCG12100.019.96142667
CTTGTGT18150.019.6721369
AGAGGTT12500.019.602765
GTCTTAC16450.019.5852221
GTATATA3100.019.2040921
CAAGAAT14400.019.20057915
GAGGTTC12600.019.16930866
AGTTAGA14250.019.16004261
TTAGAGG14600.018.94096463
Produced by FastQC (version 0.11.5)