FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334875
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57220.24506665239038494No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC54300.23256062958402485No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA53600.22956261041811662No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT49550.2122169281010761No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT48240.20660634937630495No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC47680.20420793404357834No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG41740.17876759997858557No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA38490.1648482252797259No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA37880.162235665720863No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT34000.1456180737726859No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA33220.14227742384495956No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA31880.13653835858450666No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG31020.13285507789496226No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC29620.12685903956314576No Hit
TTCTTGGACCGTCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATG27110.11610899941110339No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA26760.11460998982814927No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT25000.1070721130681514No Hit
CGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAAC23580.10099041704588041No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA23430.1003479843674715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTTT37000.024.0263588
CTTTTTT38500.022.9074739
ATCTGGG22300.022.4442847
TTTCGGA6400.022.4371071
GGTCCAA19450.022.31252511
GATCTGG51400.021.6540226
TAGACTA2001.0713848E-920.99851632
ATCTTTT47250.020.8892027
GATCTTT52050.020.3749456
GTGTAGA13100.019.50661770
CAAGAAT23050.018.97954215
CTCTCGC22700.018.80924227
TGTGTAG13400.018.8086769
ACGGTCC23250.018.6653489
CTCGCGT23300.018.62529429
ATCTGTG13350.018.6145117
AATACGC8300.018.55290421
AAGAATT24100.018.44307516
GTCCAAG23550.018.42796712
CGTCGTA47000.018.39231733
Produced by FastQC (version 0.11.5)