FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652043
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38410.5890715796350855No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT7900.12115765371302199No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA7060.10827506774859941No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA3400.032.94013270
TCCTAGT500.007203838328.0034052
AACGGTA500.00720923527.99911119
ATAGTGT4350.026.55088269
AGTTTAC750.001832522923.3325928
ATCTGTG3300.023.3325927
CGGACTT1352.312081E-723.33259215
TCTAGGC1403.2834214E-722.4992858
ATAATAG1102.9199433E-522.2737273
GTACACG800.002665225921.8759823
GTTAGAG2301.2732926E-1121.3036762
GATCTGG14500.021.2407056
TCTTTTT9250.020.8101513
CGTGTGG4050.020.74008270
ATGTCTA850.003788044420.5891633
TGTGTCC850.00378972820.5875828
GTCCTAA1205.697383E-520.423851
ATCTTTT9450.020.36972212
ATCTGGG6200.020.3219367
GGGACGT5350.020.2797316
Produced by FastQC (version 0.11.5)