FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605548
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT75170.20848425814883062No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68530.19006819490407562No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA62060.1721236272544423No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG56240.1559818368802745No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT54870.15218213708429343No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC50560.14022833699620696No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG50510.14008966182117114No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA50440.139895516576121No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT49340.1368446627253333No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC48650.13493094530983915No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT46580.1291897930633568No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA43460.12053646214112253No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT37970.10530992792219102No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG37890.10508804764213374No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA37570.10420052652190456No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA36250.1005395019009593No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA36070.10004027127083039No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTTT61900.025.50058
CTTTTTT65300.024.1188229
ATCTTTT75900.022.595567
AGGTGTA24250.021.218370
GATCTGG77000.020.6366886
ATCTGGG32700.020.5504957
TATACTA2750.020.3648015
TTTCGGA10900.019.9185141
ATCTGAG18350.019.836557
ATCTGTG16350.019.6942257
ACTTTAG5700.019.6592451
GTCTTAC27200.019.3114151
TTGTCTC25800.019.12740910
GGTCCAA22700.019.11791611
AGAAACG27800.018.76084767
TATGCTT26150.018.7391225
ATATAAG14900.018.5635172
GATCTTT96400.018.480586
AGTCTAA5700.018.4305421
CTTGTCT27000.018.2773049
Produced by FastQC (version 0.11.5)