FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949619
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC18920.19923779958067392No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA16260.17122656560157284Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC15510.16332866128415713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15110.15911644564820207No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC15050.1584846133028088No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC14660.1543777030577526Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC14530.15300873297606724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGTT501.709738E-435.00181669
TTCGGAA1800.033.0642362
TTTCGGA1850.032.1773871
CTTTTTT15850.025.1734839
TCTTTTT16450.024.0425348
ACAAGTT1902.1827873E-1123.9486169
ATCTTTT16550.023.8972637
CGAAATG2350.023.82851653
GTATAAG1052.032688E-523.3443811
GAGTGTA5250.023.33454570
TAGGAAT750.001832243623.33454561
GAGCATA1403.270743E-722.510651
GTATAGG2800.022.510651
GTCTAGG2056.002665E-1122.2056271
GTCCTAA1304.3258096E-621.5486581
CTGTACT3250.021.53844628
GATCTTT21150.021.5129856
ATATACT1807.2614057E-921.38999765
ACGAGTA2301.2732926E-1121.30545469
GAGACAA2501.8189894E-1221.0010966
Produced by FastQC (version 0.11.5)