FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378155
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26680.1935921576310357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTA2700.024.62808418
TCTTTTT20150.023.2748188
CGGTGTA10250.022.87993470
CTTTTTT20200.022.8706829
ATCTTTT22800.021.9519717
AGTCTAA800.002660525321.8847471
ATCTGGG15750.021.5557677
GGTTAGC4250.021.41042146
CGTATTC2950.021.35459321
TTTCGGA4650.021.0846581
GATCTTT27600.020.6704926
TAGGTTA850.003787740120.5914254
GTATAGT4150.020.2499791
GCGATTA2603.6379788E-1220.19104246
AATGCTA1055.4696295E-419.99874528
AGCGATT3000.019.83281155
TAGCGAT2504.1836756E-1119.59948354
GAATTAG61050.019.49382670
TACTAGC2555.4569682E-1119.2151851
GTGTAGA8950.019.16359170
Produced by FastQC (version 0.11.5)