FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611285
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26000.16136189438864013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT34050.027.3409657
AGGTGTA12650.026.8401270
GATCTTT38350.025.644276
CTTTTTT37450.024.4849289
TCTTTTT38400.023.7880388
TTTCGGA5850.021.552921
CATACGT1506.3684274E-721.006922
TAGGTGT16550.020.72675569
TTCGGAA6100.020.0885852
CGATCTT79550.019.3158635
TAGGGGT4700.018.618534
AGGGGTG13800.018.515425
GAGCGTA1953.9539373E-717.94794711
GATCTGG40150.017.608216
GCCTTAT1200.001335366617.5117451
CAGGGGT7200.017.5014214
ATACGTG4000.017.5014267
TTAGGTG19450.017.45642968
CAATACT2259.397445E-817.11254
CCAATAC3700.017.0299953
Produced by FastQC (version 0.11.5)