FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050448
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22980.2187638036342589No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACTA400.002420048734.99790629
GTCTAGG1251.05501385E-1030.8157581
TCTTTTT19000.026.3417828
ATCTTTT19150.026.3182147
CTTTTTT19050.025.9051939
GATCTTT23750.023.579786
GTCATAT750.001827479323.3452721
ATTAAGT901.9251481E-423.3397143
AGCATAC1356.009608E-620.7464122
CTATGTT850.003784550420.5938663
GAATTAG850.003788728520.58994364
TCTAGGG5300.019.8167382
TAGACTG1257.8176956E-519.6025585
TATTGAC900.005268233419.449762
CTAGACT900.00526968419.4488374
TAGTACA900.00526968419.4488374
GAGACAT2353.783498E-1019.3633166
ACCGTTA3650.019.1778478
TTTCGGA3700.018.9285981
CATACGT1501.4933628E-518.6708814
Produced by FastQC (version 0.11.5)