FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630556
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38040.2332946553200258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTTT22100.023.279919
TCTTTTT23150.022.3758898
ACGTGTA11450.022.31585770
CGTGTAG8150.021.90326170
CTAGGTC800.002666617521.8764174
ATCTTTT23850.021.7191547
CTATAAT1154.1013114E-521.3174971
GATCTTT29450.021.2734176
GTACAGG6100.020.6684881
CTATAGG2402.1827873E-1120.4292681
GTCTAGG2402.1827873E-1120.4292681
TTTCGGA4650.019.5819721
ATCTGTG13000.019.3846847
TACTATA1451.11406025E-519.3175222
TACAGTG1651.5939349E-619.0909775
GTCCTAA4250.018.952871
GACTCCT56400.018.8044170
CTACAGT2053.241803E-818.7817064
AGCATAC1702.1133637E-618.5362992
CTAGGCC1702.1214364E-618.528919
Produced by FastQC (version 0.11.5)