FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1637080
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35280.21550565641263714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG81900.030.7704265
TTCCGAT205450.030.4695242
ATCTGTG6800.030.3669727
GATCTTT37150.029.6763256
CCGATCT208500.029.6135654
TAGTGTA11450.029.34522270
TCCGATC213650.029.3128953
ATCTTTT34100.029.2515477
CGATCTA37450.027.5711655
TCTTTTT36150.027.205488
CTTCCGA237200.026.5336511
CTTTTTT37850.025.8911029
GATCTAG13350.025.4301456
GATCTGG41650.025.2935546
ATCTGAG8500.025.117097
CGATCTT66650.024.8396765
GATCTGA23250.023.4833476
GATCTAA10750.023.441346
ATCTGGG19400.023.2726787
ATCTAGC5350.022.8966877
Produced by FastQC (version 0.11.5)