FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888313
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34360.18196135916026634No Hit
TACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACG21620.11449373064740856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT32950.024.1126567
TCTTTTT33050.023.8272658
TCTAGGC3700.023.648388
CTAGGCG1802.8740033E-1023.332459
TTAGGAC5300.022.4567383
GATCTTT41800.022.2730646
CTTTTTT35400.022.1460539
TAGCACT952.7800462E-422.1079394
ATCTGGG21600.021.5512527
ACCGTTA3150.021.110878
GATCTGG55500.020.9372546
TGCGCTA4550.020.76844410
AGGTGTA11700.020.64352670
CTAGTCG11550.020.60418920
TTTCGGA5900.020.1831591
CTACTTG4000.020.1285043
TACTAAC12200.020.0955731
TAATACT1751.2649616E-720.0024224
GCCTAGG3700.019.8783261
GTCTAGG2653.6379788E-1219.8247451
Produced by FastQC (version 0.11.5)