FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917468
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC48460.2527291198601489No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT40270.21001654264895162No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC39900.20808691461865333No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC39270.20480133175625356No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC37990.19812586181360003No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA29450.15358796078995843Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA26700.1392461308350387No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG26600.1387246097457689No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT26130.1362734606262008No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC25260.13173622714955346Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT25250.1316840750406265No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA25180.1313190102781376No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC23280.12141010958201126No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC21910.11426527065901491No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA20640.10764195282528834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20110.10487789105215836No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG20110.10487789105215836No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT19300.10065357022907293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA10200.031.91430970
AGAAACG13300.022.89656367
TAGGGGT2700.022.0393684
AGAGTGT15250.022.03454269
GAGAAAC13650.021.79660866
AACGGCT14000.021.50171570
CTATAGG1656.762639E-821.2248841
GGTCCAA9600.021.14531511
GACGGAG75400.020.75099270
CTTTTTT22250.020.2921669
GAAACGG14500.020.2774868
AACGGGT15800.020.15825822
GTATAGT13250.020.0875511
AAACGGC14950.019.9012569
TATAGTA13300.019.7425352
TTTCGGA3400.019.5705911
TCAGGGT16100.019.56422638
TATGCTT10250.019.4649665
GTATTGG3600.019.4561441
TCTTTTT23400.019.4444738
Produced by FastQC (version 0.11.5)