FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340321
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATCCCCAGCACGACAGAGTTTAACAAGATTACCCAGACCTTTCGG23030.17182451069557217No Hit
GTATTACCATTGGGGCAAGGTCATCAGTAGGGTAAAACTAACCTGTCTCA22490.16779562507787313No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20770.15496287829557248No Hit
ACACTGACAGAGCCAACGAGTTCTTCACCTTGGCCGAAAGGTCTGGGTAA17240.128625903794688No Hit
GCCATAATCCAGCAGATGGTAGCTTCGCGGCAATGCCTGGTCAGACAGCC17090.1275067689008827No Hit
CTACTAGATAGTTCGATTAGTCTTTCGCCCCTATGCCCAAATTTGACGAT16780.12519389012035176No Hit
GTTTACGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACT16050.11974743363716601No Hit
TGGTAATACCGCTTAGTACGAGAGGAACCGCGGTTTCAGATAATTGGTTT15810.11795681780707755No Hit
GTTCTGGGCCGCACGCGCGCTACACTGACAGAGCCAACGAGTTCTTCACC15620.11653924694159086No Hit
GTTCTGGCATGGATTCTGACTTAGAGGCGTTCAGCCATAATCCAGCAGAT14860.11086896347964406No Hit
ATCCAGCAGATGGTAGCTTCGCGGCAATGCCTGGTCAGACAGCCGCAAAA14370.10721312282654677No Hit
CTACACTGACAGAGCCAACGAGTTCTTCACCTTGGCCGAAAGGTCTGGGT13850.10333345519468844No Hit
GACTTAGAGGCGTTCAGCCATAATCCAGCAGATGGTAGCTTCGCGGCAAT13570.10124440339291856No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGGT9850.028.7820197
GATCTGG40550.027.9678466
ATCTGGG18550.026.9814057
TGTACTA657.9543545E-426.9263655
ATTATAG808.6579385E-526.2561423
GGTATAC1207.4111085E-826.25026364
ATCTTTT21000.025.5002577
GATCTTT25900.025.1372646
GTATGTA700.001223124825.0123831
ATCTGTG9600.024.0627427
TTTCGGA2650.023.785361
CGATCTG119700.023.7163475
TCTGAGC4300.023.604018
CTTTTTT21450.023.4958659
AGGTGTA8050.023.04371370
TCTAGGC2850.022.1046648
GTATTAT800.002659718321.8858361
TTATACG800.00266548721.877674
ATCTGAG10250.021.853887
TCTGGTC5550.021.4408598
Produced by FastQC (version 0.11.5)