FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1486884
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41800.28112482211120704No Hit
GTCATAATCCCTCAGATGGTAGCTTCGCACCATTGGCCGATCGGCCAAGC35660.2398304104422403No Hit
GAGTTATCGTTGCGATAACTAGGCGGGATTCTGACTTAGAGGCGTTCAGT31140.20943126699863607No Hit
TAGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTTC23480.1579141345256254No Hit
GTACGAGAGGAACCGCAGGTTCAGACATTTGGTTCATGTGCTTGGCCGAT21720.14607729991041668No Hit
CTTCCTATCATTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCACCCA18810.12650616995004318No Hit
GACTTAGAGGCGTTCAGTCATAATCCCTCAGATGGTAGCTTCGCACCATT18800.1264389152079113No Hit
GGATTACTATTGCAACAACGAGTCATCAGTAGGGTAAAACTAACCTGTCT18220.12253814016426297No Hit
CTCTCGTACTGAGCAGGATTACTATTGCAACAACGAGTCATCAGTAGGGT17480.11756128924650477No Hit
CCCTATTGGTGGGTGAACAATCCAACGCTTGGCGAATTCTGCTTCGCAAT15970.10740582318459274No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCTGACGAT15490.1041775955622631No Hit
TCCTATCATTGCGAAGCAGAATTCGCCAAGCGTTGGATTGTTCACCCACC15210.10229446278257079No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGACTCGTTGTTGCAATAGT14980.10074760371353783No Hit
CCATTGGCCGATCGGCCAAGCACATGAACCAAATGTCTGAACCTGCGGTT14870.10000780155008729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG74900.031.3100365
CCGATCT190450.030.0354884
TTCCGAT193100.029.628282
GATCTTT23500.029.4898996
TCCGATC193900.029.4679413
ATCTTTT21700.028.708277
ATCTAGG4900.028.5700287
ATTATAT1004.413705E-728.008993
CTTCCGA207400.027.6613461
TCTTTTT22700.027.1352358
CGATCTT45700.027.0364155
CGATCTA39550.026.727055
GATCTGG34950.026.0376726
GATCTAG13250.025.8872666
CTTTTTT24050.025.612059
GATCTGA21600.025.278246
ATCTGGG16950.024.7775487
TCTAGGC3000.024.4987988
TACGTAA1451.7311322E-824.13674723
ATCTGTG6300.023.332197
Produced by FastQC (version 0.11.5)