FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1063047
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44280.41653849735712534No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT27150.029.3922257
TCTTTTT27050.028.7245458
GATCTTT30150.027.979386
CTTTTTT28250.027.7521749
CTCTAGG1253.0715582E-622.4168421
CCTAGAC1253.0816154E-622.4084033
CTGAGTG1102.9232235E-522.2726979
ATACTAT800.002655404221.8914471
TTAATAC800.002661215421.8832053
TAATACT800.002663397221.8801164
CGATCTT56100.021.7776875
TTTCGGA3400.021.6339021
TCTAGGG3450.021.3153482
TAGGACA1656.78192E-821.2170834
GAGACTT1859.944415E-920.81469766
CAATAGA1558.728348E-720.3273334
ATCTGAG6850.019.9269817
TAGGGGT4250.019.769334
TAGAGAG1451.1152397E-519.3139535
ATGGACT1651.5893584E-619.0953754
Produced by FastQC (version 0.11.5)