FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320596
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT58990.4466922510745148No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG43930.33265283250895805No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA39990.3028178186212892No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA36190.27404293213064407No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT35880.27169550718009144No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT34640.2623058073778809No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC34110.2582924679462909No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC32870.24890276814408038No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG26920.20384735377057028No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA26140.19794092970143784No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA25950.19650218537690559No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA25490.19301890964382748No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT25460.1927917394873224No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG24000.18173612520407453No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC23800.18022165749404057No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTA23430.17741989223047774No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA22670.17166491493234873No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA22430.169847553680308No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT21590.16348678929816537No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA21540.1631081723706569No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT20950.15864049262605673No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA20410.15455142980896505No Hit
GATATGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGA19630.1486450057398326No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA19580.14826638881232412No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC19530.14788777188481567No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA19270.1459189638617715No Hit
GTAGTATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTT19060.14432877276623585No Hit
GTGTTATTGTGGGCCGGTACTATTACTTTGAACAAATTAGAGTGCTTAAA18140.13736222130007966No Hit
CTAGATAACATGCAGATCGTATGGTCTTGTACCGACGACAGATCTTTCAA18010.13637781728855758No Hit
CATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAA17860.13524196650603212No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC17760.13448473265101515No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC17590.13319743509748627No Hit
GATTTAAAGTGTACTCATTCCAATTACAGGGCCTCGGATATGAGTCCTGT16690.1263823304023335No Hit
AGCTGGGAGTGGGTAATTTACGCGCCTGCTGCCTTCCTTAGATGTGGTAG16170.12244471435624521No Hit
CATTATATCAGTTATGGTTCCTTAGATCGTTAACAGTTACTTGGATAACT16120.12206609742873673No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGAGCTGGGAGTGGGTAATTTA15630.11835565153915353No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA15610.11820420476815015No Hit
AGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGT15470.11714407737112636No Hit
ATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTT15440.11691690721462127No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA15410.11668973705811618No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15350.116235396745106No Hit
TCGTTATATTGGTTGAACTCTAGATAACATGCAGATCGTATGGTCTTGTA15210.11517526934808223No Hit
CTCCCAGCTCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCATAT14990.11350935486704487No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG14980.11343363148154319No Hit
GGCTAAAACCAAGCGATCGCAAGATCGTTATATTGGTTGAACTCTAGATA14760.11176771700050583No Hit
CTATTAATCATTACCTCTTGATCTGAAAACCAATGAAAGCAGAACAGAGG14040.10631563324438359No Hit
GTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCCCCCAAACTGCT13680.10358959136632247No Hit
CGTTTATGGTTAGAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAAC13420.10162078334327834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC1004.4138687E-728.0084253
ATATAAG8750.026.4119432
GGTCCAA12600.024.44346811
TATAAGA9550.023.4625553
CTAGGCG901.9291122E-423.3332829
CGTAAAT11100.023.01622423
ACCGGTA9950.022.86254115
AACGGCT20600.022.7703970
CCGGTAA9950.022.51080916
AGAAACG20750.022.26838567
CTCGCGT13550.022.21229229
CGACGGT14050.022.1707737
AAACGGC21250.022.07388369
ACGGTCC13850.021.9855129
GTCTTAC16800.021.8915311
TAGGGGT1604.904541E-821.8782654
CGGTCCA14150.021.76591510
ATAAGAA10300.021.750824
GAGAAAC21550.021.60333666
TAACGGG22250.021.54888328
Produced by FastQC (version 0.11.5)