FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315069
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32131.01977662035935No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAT305.856279E-446.6934552
CTCAGTC350.001254759639.9975547
TTAGAGT400.002413740435.0089724
AGAGTGT400.002415621235.0034146
AAATTAG552.975739E-431.8415031
TAGAGTG552.9840693E-431.8263365
CCGCTAT552.9896334E-431.8162359
CCACTTA450.004301708731.11414164
TACGCCG450.00430505231.10920510
TCTTTTT10600.030.3754988
ATCTTTT10950.029.7242077
GATCTTT11250.029.5584376
ACTGCTC604.977964E-429.164888
CCTGTTT853.9488914E-628.8217668
TCTGTTT853.9488914E-628.82176627
GTATTGT500.00717683128.020521
GTATTGG500.00717683128.020521
ATTAGAG500.00718239928.0160713
TGTTTAG755.5777156E-527.99828729
CTTTTTT11450.027.8148929
Produced by FastQC (version 0.11.5)