FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727705
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28460.1647271959043934No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA18660.10800454938777165Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC17860.10337412926396579No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC17750.10273744649694247Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT40900.026.6995097
AGGTGTA12350.025.79045370
GATCTTT46700.025.482036
TCTTTTT42950.025.1806728
CTTTTTT43400.024.9995049
CAGGGGT6200.022.0176034
CTAGGAC2152.6302587E-919.5373233
ATCTGGG17200.018.9245917
CGATCTT105950.018.4003565
TAAGGTG4050.018.1483125
GAGGTGT17800.018.09057669
TAGGACG2155.5926648E-817.9081764
AGGGGTG18500.017.0271845
GATCTGG52500.016.6668196
GGTGTAG11400.016.57957869
TAGGGGT7250.016.4148924
GAATAGA58900.016.40130470
TTTCGGA5150.016.3198011
CGAATAG61650.015.84001969
CGTTAGG1350.00294098115.5606512
Produced by FastQC (version 0.11.5)