FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130
Sequences flagged as poor quality0
Sequence length76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCTACGTCGGGAAGGGATAAGCGCTGAAAGCATCTAAGCACGAAGCC10.7692307692307693No Hit
GTTCGGATGGGGCTTGGGGGGGGTGGGCGCGGCCGCGGGCGGGGGGGAGC10.7692307692307693No Hit
GACGTGTGCTCTTCCGATCTTGTTTGGGAGACGTGTGCTCTTCCGATCTT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAATAGTCACAACTACTCAGGAAGCAGAGGCACGCGGATCCATTGAGCCC10.7692307692307693No Hit
GTAAAGTAATGTGCTTTATTCAGAATTTTCACTAACGGATTTTTTTCCCT10.7692307692307693No Hit
CTCTTCCGATCTGCGTTGGGAGACCTGTCTCTTATACACATCTGACGCTG10.7692307692307693No Hit
GTACTATACCTTTATGTATGTAAGCGTATTACCGGTGGAGTTCTTATATG10.7692307692307693No Hit
CCATAGCAGAAAAAAGCAAACCGAATCAGGATATAGCTTGTTCTCCCCGA10.7692307692307693No Hit
GACGTGTGCTCTTCCGATCTACACAGGGAGACGTGTGCTCTTCCGATCTA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGTGCACAGTTCGCCGCGCTCGCCGACCGGCACCGTGCCGCCTTCGGC10.7692307692307693No Hit
GGTTTTTTGTTTGTTTGTTTGTTTGAGGATACCATGAGGCTTGCACATAC10.7692307692307693No Hit
GCCAAGGGAGACTACTGATCATACGATCTGCCTAGTGAGAAGTGTAATAA10.7692307692307693No Hit
CTCTTTCCCTAAAAGACGCTCTACCGAACTTACTTTCCCATGCCATTTCA10.7692307692307693No Hit
CTCTTCCGATCTGGAGTGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAA10.7692307692307693No Hit
GAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCACGGA10.7692307692307693No Hit
CGTCTCCCGGAGGAGATCGGAAGAGCACACGTCTCCCGGAGGAGATCGGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGCCGGGCCGGCGCGGGCGCGGCGCTGGGCGCGGGGGGGCTGGGTGGGG10.7692307692307693No Hit
ACGTTGGGACGTGTGCTCTTCCGATCTACGTTGGGATACGTGTGATAATC10.7692307692307693No Hit
GAAGAGCACACGTCTCCCAATCCAGATCGGAAGAGCACACGTCTCCCAAT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTCTCAATACCTATCGGTTCGACGCGGTGTTCGATTCTATTGCACGATCA10.7692307692307693No Hit
GTACTGCACGAGGGAACGTGTGGGAGAGTAAGACGCTGCCGACGATATGC10.7692307692307693No Hit
CTTGAATACTGATAGAATATCAGTTCACAAAAAAAAAAAAATAAAAATAA10.7692307692307693No Hit
TGCTTGTGTGGTGTTGTGGGAGTGTGTTGTGGCTGCCGTCGCATGCTGTT10.7692307692307693No Hit
AAACAAAACAGCATAAGACTACTTATACAATCAGGAATCTATTATGAAAC10.7692307692307693No Hit
GCACACGTCTCCCTGCACAGATCGGAAGAGCACACGTCTCCCGTCTTAGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAAAACACTACTCCCAATTAAGATCTGAACAGCACACCTATCCCAGA10.7692307692307693No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.7692307692307693No Hit
GTGGTCTTCCGATCTGGTGAGGGAGACGTGTGGTCTTCCGGTCTGGACCG10.7692307692307693No Hit
GGGGAGACGTGTGCTCTTCCGATCTGCCAGGGGAGACGTGTGCTCTTCCG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCTAGACAGGGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGG10.7692307692307693No Hit
GCAGGGCGCCGGTGTCGCAGCGGCGCTCGGGTGGGCGCCGGTGGCGCGGC10.7692307692307693No Hit
GATCGGCATATAGACCGACAGCGAGGTGATGTTGACGATGCGGCCGAAAC10.7692307692307693No Hit
CGTTGAGTAGGGTTTGCGTGGCGGTTGGGTGCGTGGGGCTATTAGGGAGG10.7692307692307693No Hit
GAAGAGCACACGTCTCCCTTGGCAGATCGGAAGAGCACACGTCTCCCAAG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AACAAACACTGTGTAAGCACTACATGTCAGACATATTTTAAGGACTTTAC10.7692307692307693No Hit
CACTCACAGTTCCCCTTAACCTTCCAGCACCGGGCAGGAGTCAGTCCGTA10.7692307692307693No Hit
GACGTCTGCTCATCCGAACTCCCAAGGGAGACGCAGTCTCTTATACACAT10.7692307692307693No Hit
GTTCCGTATCGGGCGCAGAGTTCAAGCGGCCGTTTTGCGGGCCGGGCTTG10.7692307692307693No Hit
GGGGGAAGTGAGGCCGGCAGAGAGGATGATTTTGGGGTTGGTTGTGGTGT10.7692307692307693No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC10.7692307692307693No Hit
CGTCAATGTCGGTCGACAAAGCCGTCGTTTTCGAAACGGCCGATTTCACC10.7692307692307693No Hit
AGTCAGGGAGGCGGGTGCGCGTCCGCTCGGGGATGGGGGGGGCGGTTAGA10.7692307692307693No Hit
GAAGAGCACACGTCTCCCGGAGGAGATCGGAAGAGCACACGTCTCCCGGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCAACTAGAGGCTGTTCACCTTGGAGAACTGCTGCGGTTATCAGTACGA10.7692307692307693No Hit
GCGAAGATCGGAACAGCACACGTCTCCCGGGGTAGATCTGAAGAAAACAC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATATTTTATTTTATGTTTTTTTTTTATTTTTAACAGAGGGCCCCCGCCCA10.7692307692307693No Hit
AGTTCAATTATTTTAATTTTTCGCTCACACAAATAAGTGAGAACATGTGA10.7692307692307693No Hit
CGTCTCCATGCCAGATCGGAAGAGCACACGTCTCCATGCCAGATCGGAAG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCGCGGCCCAGACCATGGCGAGGCGGGGGGTGGCGCGGGTGCGGAAATTC10.7692307692307693No Hit
CTCAAAAACAAAACGGAAGATAACAAGTCACAAAAACAAATCGAAAAAGC10.7692307692307693No Hit
GACCACTACAGGACAACATCGCAAGAGCCGACGTCACCAACACAAAATAC10.7692307692307693No Hit
CACGTCTCCCTGACCAGATCGGAAGAGCACACGTCTCCCTCGACATATCG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGCGCGCCATAGCGAGCCTGGTAGAGCCCCTCGCGCCGGCGCGGCGTCG10.7692307692307693No Hit
GAAGAGCACACGTCTCCCTTAGAAGATCGGAAGAGCACACGTCTCCCATG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGAACTTATTTTTTTTATTAATTTTTTTATTTTTAAAAAAAAACAA10.7692307692307693No Hit
CGTGTGCTCTTCCGATCTCACAGGGGAGACGTGTGCTCTTCCGATCTCAC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCTCATCCTGGGTGTAGGCGCAGCCTACGGGCTGGCGCGCACGCGCAGC10.7692307692307693No Hit
ATATGAGCCAGATCAAATCTTTTCGAGAGGAATTGGGCCAATTGCCTGCG10.7692307692307693No Hit
GCCCAGGGAGACGTGTGCTCTTCCGATCTCCTCCGGGAGACGTGTGCTCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGAGACGTGTGCTCTTCCTGTCTCTTATACACATCTGACGCTGCCGAC10.7692307692307693No Hit
GCAGTACGGGAGCCTGGAGCCGGACCAGGCGCGCGAGCTCAAGCAATTGC10.7692307692307693No Hit
TATTTATAGAGATAGAAGAGCTACACAAAAAATCCCATCAGCTAATTCCT10.7692307692307693No Hit
GATTGAAATGAGGAAGTAGTATCAGTTAGGAGAGTAGCTGTCTCTTATAC10.7692307692307693No Hit
GCTTTGGACGGATTCAGGATACATTAGGGAGAGTCCTGTAGAGAAGGGGC10.7692307692307693No Hit
GTGGTCTTAAGATCATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.7692307692307693No Hit
GATCAGGACAGGGCGGTAGGATAATAGACCCCCCAAGTGCAAGACCTTGA10.7692307692307693No Hit
ACCCATCGTACTGTGATAGGACACTGACCTATCCCACTGGGTCAATAACC10.7692307692307693No Hit
CTGCCCAGCTGGCGTACTCGCTGATATGCAGCGCCGGGTACACCCTGTCT10.7692307692307693No Hit
TCAGATCTAGAGCTGAAGACGATTTAAATTACCATCATTCCAGCGTATGA10.7692307692307693No Hit
CATCCAACGATACAACACTGGTAATAGCACCAAGGTCAATAACGTCGAAG10.7692307692307693No Hit
ACAAAATCCAGATCTCCTTCGCGTACACCTATCACGAGAAGCAGAAACAG10.7692307692307693No Hit
GGGTCGGTGCCTGCTTGCGCCGAAGGTTTTCATATGCGCTTGCATGCTCG10.7692307692307693No Hit
GTCCTCGACCGTAAAGTCCTTTTTCTTCCCGGCGACGGCGCGGATGGCGT10.7692307692307693No Hit
AGGTGAAACAGCAAAAGGAAAATAATATCGTGTAAGGAAACGTCAATCGA10.7692307692307693No Hit
GATCTAACCCGTGAACTAACTTTATATAATTTTTTATTTTTAATATATTT10.7692307692307693No Hit
GGTTGGCTCGGCTTCGGGGGATAACTTAAGCCCCGCTACATTATCGGAGG10.7692307692307693No Hit
GCGCTCGAGCGCTTGGTGGGGGTCGGCGGACCGGTAGGCGGCCCGTGCGC10.7692307692307693No Hit
TCTCAGATCGGAAGAGCACACGTCTCAGATCGGAAGAGCACACGTCTCAG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GATCACATCAGAGGAGTCGTGGGAGAGCGGCGCCAGGACGATCGCAGCGG10.7692307692307693No Hit
GGGTGAGGATCCCAAGCGCGATCTCGAGCGCGGGCTACACCTGGTGCAGA10.7692307692307693No Hit
GTCCTGGTTCCCTGCTGCCGAAACAACAGATCACCAAGAGGAAAGACCCA10.7692307692307693No Hit
ATCTCACCTGGGAGACGTGTGCTCTTCCGATCTGCAATGGGAGACGTGTG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCATTATGTCAACGGTGGTGACTTCGGCAACCGCGAGGACCAGATCAACC10.7692307692307693No Hit
GGGCCCACGTGTCGGATGGTAGTTCGGATGGGGGGGGGTCGGGGGGGGGG10.7692307692307693No Hit
GCGCTTGACCTTGCCGCTGGCGCCCTTTGGCAGCGCGGCCACGCTGCGGA10.7692307692307693No Hit
TAACACCACAACAATAAATATGGAGAACATAACAGAACACGACTACAAAT10.7692307692307693No Hit
GTGCTCTTCCGATCTGCTTAGGGAGACGTGTGCTCTTCCGATCTGCTTAG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTAGAGTATACCGAGGCGATCGAGTGAATCGTGGTGAAGGAACTCGGCA10.7692307692307693No Hit
GGGTGAAGGGGATTTATGCAGCCCGGCTGCGGTCTGCCCGGGTGACACAG10.7692307692307693No Hit
GAGTACTACGGGGGAGACTGGAATGTCGGGGGGAGGGGTGGAAGGCACAG10.7692307692307693No Hit
CCATGGAGTAGTGAGCCATGCCGGTGGCTCCAGCCAGAAGCGAGCTAAAT10.7692307692307693No Hit
CTTCCGATCTGTGCTGGGAGACGTGTGCTCTTCCGATCTGGGCTGGGAGA10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGGATAGGGGAGTTAAGAGGTGTGTGGTGTCATATGGGCCGCGTAGTTAA10.7692307692307693No Hit
GACCTAGGTGAGGACAGGCATTTTTGTTTTCCTGCCCAAATGTTGCATTT10.7692307692307693No Hit
CCTCAACCAAGATAGCAATGAACAGAACACCTATCCAAGCCTAGAACATA10.7692307692307693No Hit
CATCCGATCAGCACACGTATACGAGTTCACATACGAACAGCACAGGGAGA10.7692307692307693No Hit
AGAAAACAAATATCCATATCACTCCAAAAAGCGAACAAAAAGACAAACTC10.7692307692307693No Hit
GTAATAGTACCGGCCCACAATAACACTCGTTTAAGAGCACTAATGCAGGT10.7692307692307693No Hit
CGTATATTAAGATCACACGACTCGCAAGTTCCCGGAACACATAAGCTAAC10.7692307692307693No Hit
GGAGACGTGTGCTCTTCCGATCTGTCCCGGAGACGTGTGCTCTTCCGATC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCTCTTCCGATCTAGCACGGGGTGCTCTTCCGAAAAAAAAAAAAAAAA10.7692307692307693No Hit
CAATATCCGCGTGAACGGCGAAGTCGCGCAGAGCTTCGAGAATGGCTTCG10.7692307692307693No Hit
AATCCAATCTATGCATTAATTAGATAAAACCAAGAACCAGCCAATCCCCG10.7692307692307693No Hit
GTGCAATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGCGAAC10.7692307692307693No Hit
TGATATATATGTAGTTGTGTTGGGGTATGGGGTGGGGGCGTGGGGCTGGG10.7692307692307693No Hit
CCCAGGGAGTGCTCTTCCGAGACGTGTGCTCTTCCGCGACGCTGTCGGTT10.7692307692307693No Hit
CCCGTACGAGATCGGAAGAGCACACGTCTCCCGTACGAGATCGGAAGAGC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCTTCACTGATGAAGACGCGATAGGTCTGCGTCCCCAGCGGCTTGCCGAT10.7692307692307693No Hit
CTGCGGGTCGGTTGTTTTTTTGTTGTTGTTGTTTGTGGGGGGGGGGGGGG10.7692307692307693No Hit
TACCCTCGATATGCGAATCGCTCAAGTCAATGCCCCTGAGCGGAGTATGC10.7692307692307693No Hit
CTGCTCGCGCTGACGCTCGTATTCGGTGCGTACTCCTTCGTCGACGGCGC10.7692307692307693No Hit
CCAGTACTTTCAATACACCTTGATCGGCTTACAGAACGCTCCCCTAACAC10.7692307692307693No Hit
GACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTT10.7692307692307693No Hit
CGTGACCAATTCCTAAAAAATCTGTCAGCCATCAACTTCACCATACTCAA10.7692307692307693No Hit
GTGCTCTTCCGATCTGGTGGGGGGCCAGCGGGTCGAAGACGGCGCCGGTC10.7692307692307693No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAA10.7692307692307693No Hit
CACGTCTCCCAGTCCAGATCGGAAGAGCACACGTCTCCCAGTCCAGATCG10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTAGTCCCAGGGTTCCAGGGGGAGGCCCAAACCTTCCGGCATCTTCCCC10.7692307692307693No Hit
CATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGCTGTCTCTT10.7692307692307693No Hit
GTGCTCTTCCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT10.7692307692307693No Hit
GGGGGGTTGGGATGTTGGGGGTGGTGCATTGTTTCCGGCTGGCGGCAGGG10.7692307692307693No Hit
CGTCTCCCTGTGAAGATCGGAAGAGCACACGTCTCCCTGTGACTGTCTCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCGGGGGAGATCGGAAGAGCACACGTCTCCCAGCTCAGATCGGAAGAGC10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAAAA10.7692307692307693No Hit
GTCTTTAGATCACTGTGCTCTTCATATCATTCAAGGCAGACATCGGCTCT10.7692307692307693No Hit
GAAAAGGTAGACGTGTGCTCTTCCGATCTGTAAAGTGAGACGTGTGCTCT10.7692307692307693Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTGCCGATCTGGGGTGGGGATATGAGGAGGGTTTTTGGTTTTTTTGTTAT10.7692307692307693No Hit
CTTCCGATCTTATTTTTTTTTTATTTTTTTTTTTTAGAGAAATGTTTTAA10.7692307692307693No Hit
ATTCCAAACTTTTATATAATAAATAAAAAATAAAATAAAACATAGAAAAC10.7692307692307693No Hit
GACCACATACCCTCATTATAAATTTTTTTTTTTTTTTATTTATTTTAATT10.7692307692307693No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers