FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1237010
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA3750.032.6761270
TAAGCGG552.9956055E-431.81452810
GATCTGG16750.024.8658316
GGACGGG1451.7318598E-824.13515912
CGATCTT20050.023.04725
ACGGTGT5800.021.12680469
ACCGATA1003.9369182E-420.99758519
CGATCTG61700.020.8795575
ATAACGG850.00378634920.592535
TGAGCGG1205.723205E-520.4143210
GTATTAT3300.020.1744791
CTAAGCG900.00528059419.4422119
TCACTCG900.00528059419.44221140
TTATACT4600.019.0280134
CCGATAA950.007215686618.41893652
CCGCTCT950.007215686618.41893623
CCGATCT153500.018.3611034
TCCGATC159100.017.8504753
GACGGTG8450.017.81580570
TTCCGAT159850.017.7717532
Produced by FastQC (version 0.11.5)