FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768351
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35220.1991686039705918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT37400.025.8290237
TCTTTTT37500.025.106818
GATCTTT45100.023.8249686
CTTTTTT40150.023.0996959
TAGCACT1604.902722E-821.8800744
TTGTGTA12600.021.66801570
GAACGTA2603.6379788E-1220.1907118
TATGTAC900.00526917219.4500543
GATCTGG55500.018.8559636
GATACTC56700.018.4579470
TCTGGTC11100.017.9730748
TCTGACC8500.017.7059828
TTCTATA1401.8622814E-417.505052
GTTACAC1602.6022433E-517.5050493
ATCTGGT17300.017.3989437
TAGGGGT5250.017.3373534
CTTGTGT15500.017.16284469
TTACACT2259.382893E-817.1150784
CGATCTT99400.017.113745
CGATTAT1854.7473077E-617.02904965
Produced by FastQC (version 0.11.5)